SILAC and iTRAQ datasets identify common proteins differentially expressed on the plasma membrane of SD1 cells. (A) Summary of SILAC and iTRAQ workflows. (B) Proportionate Venn diagrams showing overlap in identification of proteins between SILAC, iTRAQ, and all datasets. (C) Western blot analysis of plasma membrane fractions of proteins identified as differentially expressed in SD1 cells compared with both REH and SupB15 by SILAC and iTRAQ. CD81, CD38, CD19, and CD10 were underexpressed by SD1 in both SILAC and iTRAQ. CD48, RHOG, CD44, and RAC2 were overexpressed by SD1 in both SILAC and iTRAQ, and CD31 was identified to be overexpressed in only the SILAC data. All lanes were loaded with equal amounts of protein. Because these are plasma membrane extracts, traditional loading controls were not available. However, CDC42 and GNAI2 proteins were identified as equally expressed in all 3 cell lines by MS and Western analyses. (D) Flow cytometric analysis confirmed that CD44, CD70, ICAM1, and LFA-1 were overexpressed and CD38, CD10, and CD19 were underexpressed in SD1 compared with REH and SupB15 cells.