α4β7 and CCR5 form complexes on Vγ2Vδ2 T cells. (A) Flow cytometry assay for expression of CCR5 and β7 on Vγ2Vδ2 T cells. (B) Vγ2Vδ2 T cells were stained with β7 mAb (green) and CCR5 mAb (red), and viewed under a confocal microscope. Yellow in the merged panel represents the colocalization of CCR5 and β7. (C) Vγ2Vδ2 T cells were treated or not with the crosslinking reagent DTSSP followed by coprecipitation with protein G magnetic beads and the rat IgG2a+DTSSP (lane 1), β7 mAb FIB27 (lane 2), β7 mAb FIB27 + DTSSP (lane 3). Cell lysates were run as a positive control (lane 4). CCR5 and β7 were detected by Western blot with specific antibodies. Data are representative of 3 independent experiments.