American Society of Hematology

Figure 1

$Tie2-Cre;Ezh2fl/fl embryos die at mid-gestation. (A) Appearance of Tie2-Cre and Tie2-Cre;Ezh2fl/fl embryos at E12.5. (B-D) Absolute cell numbers of whole fetal liver at E12.5 (Tie2-Cre;Ezh2, n = 27; Tie2-Cre;Ezh2fl/fl, n = 32) (B), KSL cells per fetal liver at E12.5 (Tie2-Cre;Ezh2, n = 11; Tie2-Cre;Ezh2fl/fl, n = 15) (C), and myeloid progenitors per fetal liver at E12.5 (Tie2-Cre, n = 10; Tie2-Cre;Ezh2fl/fl, n = 6) (D). Data are mean ± SD. (E) FACS profiles of erythroid differentiation in Tie2-Cre;Ezh2fl/fl fetal livers at E12.5. Representative FACS profiles are shown. (F) Absolute numbers of erythroblasts in the fractions defined by expression of CD71 and Ter119. Data are mean ± SD (Tie2-Cre, n = 3; Tie2-Cre;Ezh2fl/fl, n = 7). **P < .005.$

Tie2-Cre;Ezh2^fl/fl embryos die at mid-gestation. (A) Appearance of Tie2-Cre and Tie2-Cre;Ezh2^fl/fl embryos at E12.5. (B-D) Absolute cell numbers of whole fetal liver at E12.5 (Tie2-Cre;Ezh2, n = 27; Tie2-Cre;Ezh2^fl/fl, n = 32) (B), KSL cells per fetal liver at E12.5 (Tie2-Cre;Ezh2, n = 11; Tie2-Cre;Ezh2^fl/fl, n = 15) (C), and myeloid progenitors per fetal liver at E12.5 (Tie2-Cre, n = 10; Tie2-Cre;Ezh2^fl/fl, n = 6) (D). Data are mean ± SD. (E) FACS profiles of erythroid differentiation in Tie2-Cre;Ezh2^fl/fl fetal livers at E12.5. Representative FACS profiles are shown. (F) Absolute numbers of erythroblasts in the fractions defined by expression of CD71 and Ter119. Data are mean ± SD (Tie2-Cre, n = 3; Tie2-Cre;Ezh2^fl/fl, n = 7). **P < .005.

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