Vegfa expression in ROS^lo and ROS^hi HSC populations. BM cells from Vegfa^δ/δ or WT mice were stained with markers to detect LSK CD34 and DCFDA to detect ROS. (A) Representative FACS profiles of LSK gated cells showing gates used for sorting of CD34⁻ ROS^lo and CD34⁻ ROS^hi populations. (B) Percentage LSK CD34⁻ ROS^lo and LSK CD34⁻ ROS^hi cells within LSK gated cells. Bars represent mean ± SD. P values are from t test. (C) Vegfa expression in sorted subpopulations. Each dot represents an individual mouse and connecting lines are between littermate pairs. P values are from paired t test. Results shown are data combined from 2 independent experiments.