Pre-NKPs and rNKPs can be highly purified from BM using 12-color flow cytometry. (A) Gating strategy for CLP, pre-NKP, and rNKP. (B) Frequency of CLP, pre-NKP, and rNKP in BM isolated from 2 femurs. (C) Absolute number of CLP, pre-NKP, and rNKP from 2 femurs. Shown are the individual data from 10 mice with the mean and standard deviation (B-C).