MTOC and perforin granules do not polarize to the synapse of autologous mature DCs with resting NK cells. Co-cultures of mature DCs with resting NK cells were fixed after 1 or 20 minutes of interaction. F-actin (green), nuclear DNA (blue) and α-tubulin or perforin (red) were stained. Arrows indicates MTOC (top) and perforin granules (bottom) of NK cells. The number of conjugates with MTOCs or perforin granules distant from the synapse was plotted in the graph as percentages of total number of conjugates analyzed. Original magnifications are 100× for all the microscopy images. A total of at least 200 conjugates from 3 independent experiments were analyzed. Values on graph bars are medians and error bars represent interquartile ranges. *P < .05. Statistics were performed with a null value of 75%, representing random distribution. Scale bars are 10 μm.