Decreased inflammatory response in Tmprss6^−/− mice.Tmprss6^−/− mice, maintained at a regular diet, and control littermates, fed an ID diet, were injected with LPS and then killed 6 hours later (3 mice for each group). Hepatic expression of hepcidin (Hamp; A) and A2M (B) analyzed by TaqMan qRT-PCR. Analysis of leukocytes recruitment (CD45⁺; C) and infiltrating macrophages (CD11b⁺/F4/80⁺; D) in liver of Tmrpss6^−/− and control mice. Liver (E) and spleen (F) IL6 mRNA expression were analyzed by TaqMan qRT-PCR. Hprt1 was used as the housekeeping gene to normalize gene expression. mRNA expression ratio was normalized to an ID mean value of 1. Error bars indicate SE; ns, not significant; *P < .05; **P < .005; and ***P < .0001.