Restoration of intestinal transepithelial dendrite formation. (A-B) Representative confocal image of small intestinal villi demonstrating TEDs formed by CX₃CR1/GFP⁺ lamina propria macrophages in panel A (CX₃CR1^gfp/+ > WT) and panel B (CX₃CR1^gfp/+ > CX₃CL1^−/−) BM chimeras after Aspergillus conidia challenge (1-2 × 10⁸ conidia/mL). Green represents lamina propria macrophages; red, conidia; and yellow, merge. Arrows indicate TEDs. (C) Bar diagram summarizing TED quantification in indicated BM chimeras. Note the absence of extension in mice with impaired CX₃CR1/L1 interactions. Only TEDs that reached the lumen were considered for the quantification. Results are 1 representative of 3 experiments (N = 4). Error bars represent SD. n.d. indicates not detectable. (D-E) Representative confocal image of small intestinal villi demonstrating TEDs formed by CX₃CR1/GFP⁺ lamina propria macrophages in panel D (CX₃CR1^gfp/+ > CX₃CL1^395AA:CX₃CL1^−/−) and panel E (CX₃CR1^gfp/+ > CX₃CL1^105Δ:CX₃CL1^−/−) BM chimeras after Aspergillus conidia challenge. Arrows indicate TEDs. (F) Bar diagram summarizing TED quantification in indicated BM chimeras. Note the restoration of extensions in CX₃CL1^−/− mice harboring the CX₃CL1^395AA and CX₃CL1^{105 Δ} transgenes. Results are one representative of 3 independent experiments (N = 3-5). Error bars represent SD.