American Society of Hematology

Figure 3

$Figure 3. AML1 inhibits nuclear translocation and phosphorylation of p65. (A) Nuclear translocation assays of p65 in HEK293T cells transduced with AML1 or mock, 20 minutes after the addition of TNF-α. Scale bar represents 10 μm. Green indicates AML1; blue indicates TO-PRO3 (nucleus); and red indicates p65. (B) Intracellular FACS analysis of phospho-p65 (Ser 536) in HEK293T cells transduced with AML1 or mock. GFP-positive fractions were gated. Green line shows phosphorylated p65 protein 5 minutes after TNF-α stimulation. MFI indicates mean fluorescence intensity.$

AML1 inhibits nuclear translocation and phosphorylation of p65. (A) Nuclear translocation assays of p65 in HEK293T cells transduced with AML1 or mock, 20 minutes after the addition of TNF-α. Scale bar represents 10 μm. Green indicates AML1; blue indicates TO-PRO3 (nucleus); and red indicates p65. (B) Intracellular FACS analysis of phospho-p65 (Ser 536) in HEK293T cells transduced with AML1 or mock. GFP-positive fractions were gated. Green line shows phosphorylated p65 protein 5 minutes after TNF-α stimulation. MFI indicates mean fluorescence intensity.

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