IL-36Ra acts as a selective inhibitor of IL-36α, IL-36β, and IL-36γ in BMDCs. BMDCs were pretreated or not with 10 ng/mL, 100 ng/mL, or 1 μg/mL IL-36Ra for 20 minutes before the addition of IL-36α (A), IL-36β (B), IL-36γ (C), or IL-1β at the indicated concentrations. After 72 hours, IL-6 levels in cell supernatants were determined by ELISA. *P < .05 versus IL-36α alone (A), IL-36β alone (B), or IL-36γ alone (C). Data are shown from 1 of 3 independent experiments with similar results. Data are mean ± SD of culture triplicates in the same experiment.