Irgm1^−/− HSC function is rescued by the addition of homozygous disruption of Irgm3. (A) The absolute number of SP^L cells per mouse is indicated. Data are representative of 2 experiments, each performed in triplicate. (B) Competitive BM were performed with the use of 2 × 10⁶ WBM cells from CD45.2 WT, Irgm1^−/−, Irgm3^−/−, or Irgm1^−/−Irgm3^−/− donors admixed with 2 × 10⁶ WBM competitor cells from CD45.1 WT mice. Data are representative of 2 independent experiments, each with 5-6 recipients per cohort. (C) LC3 aggregation was determined by immunohistochemistry in HSCs (CD150⁺ SP^KLS) of WT, Irgm1^−/−, Irgm3^−/−, or Irgm1^−/−Irgm3^−/− mice. A minimum of 40 cells was assessed per genotype, and the percentage of cells with LC3 aggregation is shown in the bar graph. Data are representative of 2 independent experiments. The proportions are statistically different with 95% confidence according to a 2-sample test of proportions. ***P < .001.