JAK2 inhibition induces durable tolerance to alloantigen but preserves antiviral immunity. Graphs show responder T-cell proliferation measured by colorimetric assay after: (A) primary allogeneic MLR to which TG101348 or DMSO diluent control was added (5 days), followed by restimulation of T cells by first-party allogeneic moDCs (3 days); (B) unadulterated primary MLR (5 days), after which T cells were restimulated by first-party allogeneic moDCs in the presence of TG101348 or DMSO diluent control; or (C) T-cell responders from a primary allogeneic MLR to which TG101348 or DMSO diluent control was added as in panel A, stimulated de novo against autologous HLA-A*0201–expressing moDCs loaded with fluMP (5 days). Scatter plot (left) and bar graph (right) show replicate means ± SD from 4 independent experiments; *P < .05; **P = .001-.01; NS, not significant; the permutation-based paired t test was used in panels A and C, and the paired t test was used in panel B. Stimulation indices were calculated as in Figure 2C and F.