Effect of PKK and fXII ASOs on FeCl₃-induced inferior vena cava thrombosis. Male BALB/c mice were treated with either PKK (A) or fXII (B) ASO at indicated doses administered subcutaneously for 3 weeks (n = 8 per treatment group). FXI ASO treatment was used as a reference antithrombotic drug (B). Thrombosis was induced by 3 minute exposure of the inferior vena cava to a 10% FeCl₃ solution and assessed by RT-PCR measurement of platelet factor 4 (PF4) mRNA levels at the site of injury. Comparison of effects of PKK and fXII protein inhibition in FeCl₃-induced vena cava (IVC) thrombosis (C). *P ≤ .05, **P ≤ .01 and ***P ≤ .001 compared with untreated control.