Blood loss after tail clip in HB mice following FXa-I16T administration. Five minutes before injury, FXa-I16T was given to HB mice via tail vein at the indicated dosage (blue bars). In some experiments, mice received FXa-I16T 14-56 μg/kg with FVa 400 μg/kg (hatched bars). Control experiments include HB mice treated with PBS (white bar), wt-mice (black bar) injected with PBS, and HB mice injected with FVa 400 μg/kg (red bar). Total blood loss (µL) was measured following tail transection. The number of animals per group is indicated, and all measurements are presented as mean ± SEM. For statistical comparisons, HB animals injected with different doses of FXa-I16T and wt-mice are compared with HB-PBS controls: **P < .001; HB animals injected with different doses of FXa-I16T along with FVa are compared with FXa-I16T-injected HB: *P < .05.