Ex vivo assessment of FXa-I16T-Thrombin generation assay. (A-B) HB mice (n = 5-7 per group) were administered FXa-I16T (450 μg/kg) and at various time points (10, 30, 60, and 120 minutes) and blood was collected and processed for thrombin generation assay. The assay was performed in either (A) the absence or (B) the presence of added FVa (20 nM). (C-D) HB mice (n = 5-7/group) were administered different doses (28-450 μg/kg) of FXa-I16T (C) alone or (D) together with FVa (200 μg/kg). After 10 minutes, blood was collected and processed for thrombin generation assay. For comparison purposes, data from (C) (28 and 56 μg/kg FXa-I16T) are provided in (D). Control experiments include (A) wt-mice (n = 5), (C) HB mice injected with PBS (n = 5), and (D) mice administered only FVa 400 μg/kg (n = 3). Data are presented as mean ± SEM.