Absence of FLT3 and NPM1 mutations in MSCs. (A) The DNA sequence derived from the HCs of patient 1093 demonstrates mutation of the NPM1 gene. (B) MSCs of patient 1093 display the wild-type NPM1 gene. (C) The FLT3-ITD mutation was detected in the HCs of patient 0757 (lane 2), but not in the MSCs (lane 3). Lane 4 displays the negative control, lane 5 the positive control, and lanes 1 and 6 the size standards. (D) The FLT3-D835 mutation was observed in the HCs of patient 0281 (lane 1), but not in the MSCs (lane 2). Lanes 3 and 4 present the positive control and size standard, respectively.