Results of FISH of HCs and MSCs. FISH of HCs and MSCs from a patient (N0214) with s-AML M6 (A-D). (A) FISH image with probes LSI EGR1 (5q31, red)/D5S23 (5p15.2, green) showing del(5q) in interphase nuclei from leukemic blasts. (B) FISH image of MSCs with the same probes demonstrates 2 signals for chromosome 5. (C) FISH image with probes D7S740 (7q22, green)/(7q35, red) demonstrates del(7)(q22) in MSCs and 2 signals for corresponding chromosomes in HCs (D). FISH of HCs and MSCs of a patient (N1150) with s-AML M4 (E-J): (E) FISH image with probes LSI WBS (7q11, red) and LSI D7S522 (7q31, green) demonstrates monosomy 7 in HCs. (F) MSCs shows 2 signals for chromosome 7. (G) MSC metaphase with 1 split signal from MLL DC probe confirms del(11)(q23). (H) Interphase nuclei of HCs demonstrate 2 normal chromosomes 11.