MML-specific cmASCs are altered after SIV infection. ELISpot analysis of classic memory (CD27⁺) B-cell Ig responses (cmASCs). (A-B) Specific for MML or (C) total Ig (heavy and light chain, positive control). (A) Number (per 10⁶ cells) of MML-specific cmASCs 2 weeks after neo MML vaccination for IgG (left), IgM (center), or IgA (right). (B) Number (per 10⁶ cells) of MML-specific cmASCs 2 weeks after recall MML vaccination for IgG (left), IgM (center), or IgA (right). (C) Number (per 10⁶ cells) of total Ig cmASCs 2 weeks after neo or recall MML vaccination for IgG (left), IgM (center), or IgA (right). SIV⁻ RMs (circles) and SIV+ RMs (squares). P values represent Mann-Whitney U t test results, horizontal bar represents median. For one animal (CE5D), we did not have sufficient cells to perform the IgA assay.