HOCl preferentially oxidizes M1606 in larger VWF multimers. Plasma VWF (5 μg/mL) was oxidized under shear stress as in Figure 2. Oxidized VWF in the reaction mixture was separated on a size-exclusion column into fractions containing predominantly high- (6), medium- (8), and low- (10) molecular-weight VWF multimers. (A) VWF multimer gel of the 3 fractions. (B) Percent oxidation of M1606 in the different fractions. Results represent the means ± SD of 2 experiments, with duplicate LC–MS/MS analyses.