mRNA and protein expression level of α-granule contents in WT and ki/ki mice. (A) Relative expression levels of mRNA for VWF, PF4, PBP, and P-selectin (P-sel) from FL-derived megakaryocyte total RNA isolated from WT and sibling FOG1 ki/ki mice. Open bars represent data from microarray analysis; and closed bars, quantitative RT-PCR analyses of similar samples. Data are mean ± SD. For microarray, N = 3; for quantitative RT-PCR, N = 7, each measured in duplicate. *P < .05, for microarray dataset. **P < .01, for quantitative RT-PCR dataset. (B) Representative Western blot analysis for α-granule proteins in both FL-derived megakaryocytes and isolated platelets from FOG1 ki/ki mice and sibling WT mice. β-actin was probed as a loading control.