TACI deficiency decreased serum IgM, IgG, and IgA and impaired antigen-specific IgM production in response to a protein antigen. (A) Sera IgM, IgG1, IgG3, IgG2b, and IgA quantified by ELISA. Sera were obtained from nonimmunized C57BL/6-TACI-wt (TACI-wt) or C57BL/6-TACI-ko (TACI-ko) mice. Concentrations were calculated by comparison with standard curves obtained with known concentrations of the respective isotypes. (B-C) Concentrations of NP-specific IgM (B) or IgG (C) determined by ELISA in the sera of C57BL/6-TACI-wt (TACI-wt) or C57BL/6-TACI-ko (TACI-ko) mice immunized with 100 μg NP-OVA 21 days earlier. TACI deficiency decreased NP-specific IgM but not NP-specific IgG. Concentrations were calculated by comparison with standard curves obtained with known concentrations of NP-specific monoclonal antibodies. (D-E) Concentrations of NP-specific IgM (D) or IgG1 (E) determined by ELISA in the sera of QM-TACI-wt or QM-TACI-ko mice after immunization with 100 μg NP-OVA. TACI deficiency decreased NP-specific IgM production and accelerated the production of NP-specific IgG. Concentrations were calculated by comparison with standard curves obtained with known concentrations of NP-specific monoclonal antibodies. Each dot represents results obtained from one mouse. Averages were compared by t test. Significant differences are noted: *P < .05; **P < .01.