Fewer colon TS1 proliferate in T_EM than in T_N recipients. Mice were transplanted as in Figure 5. On days 14, 21, and 28, mice received BrdU 30 minutes before sacrifice. (A) Representative BrdU staining on CD4⁺TS1⁺ cells recovered from colon on day 14. The no BrdU control depicts a recipient of TS1 T_N and T_EM that was not injected with BrdU. Percentages of colon (B) and splenic (C) CD4⁺TS1⁺ cells that are BrdU⁺. For each time point, data were combined from 2 independent experiments with similar results.