A TCR Tg model of GVHD. (A-B) HA104 mice received 750cGy and 8 × 10⁶ BALB/c RAG^−/− BM cells alone or in combination with 9000, 3000, or 1000 sorted TS1 T_N. As an additional control, wt mice received BM cells in combination with 3000 T_N. Shown are survival (A) and weight loss (B). P values are relative to BM only controls. Data are representative of 2 independent experiments. (C-D) HA104 mice received 750cGy and 8 × 10⁶ RAG^−/− BM cells alone or in combination with 3000 sorted TS1 T_N. On day 21, mice were killed and tissues were taken for histopathology analysis. Representative skin, colon, and liver pathology are shown in panel C. Pathology scores are shown in panel D. Pathology data were combined from 2 independent experiments.