Figure 3
Figure 3. Immunophenotype of PU/ER(T)+/− heterozygote mice. (A) BMDMs from heterozygote PU/ER(T)+/− and WT mice were subjected to flow cytometry analysis for the presence of CD 11b, F4/80, ER-MP58, and Ly-6C antigens. (B) EBA (2%) was injected retro-orbitally in to anesthetized PBS and LPS-treated WT and PU/ER(T)+/− mice. After 30 minutes, mice were euthanized, and lung vascular permeability was determined by quantifying EBA extravasation and (C) left lung wet/dry weight ratio. Data represent mean ± SEM of 4 individual measurements (*P < .01 compared with WT LPS treatment, **P < .001 compared with PBS-injected controls). (D) Survival of PU/ER(T)+/− and WT mice after intraperitoneal injection of LPS (40 mg/kg) was monitored at regular time intervals up to 72 hours (n = 10).

Immunophenotype of PU/ER(T)+/− heterozygote mice. (A) BMDMs from heterozygote PU/ER(T)+/− and WT mice were subjected to flow cytometry analysis for the presence of CD 11b, F4/80, ER-MP58, and Ly-6C antigens. (B) EBA (2%) was injected retro-orbitally in to anesthetized PBS and LPS-treated WT and PU/ER(T)+/− mice. After 30 minutes, mice were euthanized, and lung vascular permeability was determined by quantifying EBA extravasation and (C) left lung wet/dry weight ratio. Data represent mean ± SEM of 4 individual measurements (*P < .01 compared with WT LPS treatment, **P < .001 compared with PBS-injected controls). (D) Survival of PU/ER(T)+/− and WT mice after intraperitoneal injection of LPS (40 mg/kg) was monitored at regular time intervals up to 72 hours (n = 10).

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