Temporal profile of monocytes and monocyte-derived macrophages during resolving inflammation. (A) Cells from the naive peritoneum were labeled with Ly6c and F4/80, which identified 2 regions (i-ii) by FACS. Each region was further probed for the expression of CD11b, 7/4 antigen, MHC class II, CD86, CD62L, CD11c, CD115, and CD209. A similar approach was used on samples obtained from (B) 24 hours, (C) 48 hours, and (D) 72 hours resolving inflammation as well as (E) inflammation at 72 hours triggered by 10 mg zymosan. Representative figures of 4 replicates are shown from each time point.