Neutrophil degranulation increases TNF-α–mediated IL-12 expression in DCs. (A) Supernatant TNF-α accumulation of vehicle-treated, cytochalasin B-treated, genistein-treated, or genistein + cytochalasin B–treated POD1 airway neutrophils from Ext CI Balb/c → B6 lung recipients. Results are representative of 3 independent experiments. (B) Supernatant IL-12 accumulation in BMDC cocultures with vehicle-treated, cytochalasin B-treated, genistein-treated, or genistein + cytochalasin B–treated POD1 airway neutrophils from Ext CI Balb/c → B6 lung recipients cultured in the absence or presence of TNF-α Abs. Results are representative of 3 independent experiments. Data for panels A and B represent the mean ± SEM. *P < .05; **P < .01.