Emergency granulopoiesis. (A) PaO2 (left) and Evans Blue Dye (EBD; right) exclusion in sham-operated B6 mice or 24 hours after Min CI or Ext CI Balb/c → B6 transplantation (N ≥ 5). (B) Histology from Min CI (n = 4) or Ext CI (n = 5) Balb/c → B6 lungs on POD 1. (C) Serum concentrations of indicated cytokines from Min CI or Ext CI Balb/c → B6 transplantations 6 and 24 hours after engraftment (n ≥ 4). (D) Neutrophil numbers in the blood, graft tissue, and BAL from Min CI or Ext CI Balb/c → B6 transplantations on POD 1 (n = 4). (E) BM cells were isolated from Min CI or Ext CI Balb/c → B6 lung transplantations 36 hours after engraftment and 2.5 × 104 cells per plate were assayed in methylcellulose for CFU (left), total number of cells per plate to CFU (center), and CFU (right) differentiated as arising from granulocyte (G), granulocyte-macrophage (GM), and granulocyte-erythroid-macrophage-megakaryocyte (GEMM) progenitors. Data shown are from 1 representative experiment from 4 independently conducted experiments. Data for panels A through E represent the mean ± SEM. *P < .05; **P < .01.
