Chronic Rps19 deficiency results in bone marrow failure. (A) Bone marrow cellularity (n = 9-14 per genotype) and hematopoietic stem and progenitor cell frequencies in transgenic mice after 10 days of doxycycline administration (n = 18-31 per genotype). (B) Erythroblast frequencies after 10 days of doxycycline administration (n = 11-18 per genotype). (C) Bone marrow cellularity (n = 10-12 per genotype) and hematopoietic stem and progenitor frequencies in transgenic mice after 7 weeks of doxycycline administration (n = 10-17 per genotype). (D-E) shRNA-B mice (CD45.2; n = 3-5 per genotype) were induced for 30 days followed by 15 days without doxycycline, and unfractionated bone marrow cells were transplanted into lethally irradiated wild-type recipients (CD45.1/CD45.2; n = 3-5 per donor) together with an equal number of wild-type competitor bone marrow cells (CD45.1). (D) Average donor reconstitution 1 and 4 months after transplantation. (E) Lineage distribution of donor-derived cells in peripheral blood 4 months after transplantation. (F) Donor HSC frequencies in bone marrow at the time of transplantation. Error bars represent SD.