Rps19 knock-down mouse models show a reduction in Rps19 expression on doxycycline administration. (A) Overview of modified loci. (B) Breeding strategy to adjust the level of Rps19 down-regulation. (C) Quantitative real-time PCR analysis of Rps19 mRNA levels in erythroid (preCFU-E/CFU-E), myeloid (preGM/GMP), and multipotent (LSK) hematopoietic progenitors from adult mice that were given doxycycline for 3 days. (n = 4-7 per genotype). (D) Representative polysome profiles of cells derived from the liver after 10 days of doxycycline administration. (E) Pre-rRNA analysis of heterozygous shRNA-B embryonic fibroblasts (EF) cultured for 7 days with or without doxycycline (n = 4), or of cells derived from the liver after 10 days of doxycycline administration. (n = 6-10). Error bars represent SD. SA indicates splice acceptor; and pA, polyadenylation signal. Black arrowheads in panel A indicate transcriptional start sites.