CD132 mAb treatment attenuates characteristics of chronic GVHD. (A) Collagen deposition was quantified by trichrome staining in the lungs and liver. In both organs, the accumulated collagen was reduced when mice were treated with anti-CD132 mAb compared with mice treated with the isotype (*P < .05, **P < .01). The trichrome area ratio equals (trichrome area)/(total area). (B) Germinal center B-cell hyperplasia was assessed in the spleen using a PNA stain (*P < .05, **P < .01, ***P < .001). (C) PFTs were performed in mice developing chronic GVHD to observe lung resistance, elastance, and compliance (**P < .01, ***P < .001). (D) Ig deposition (green) is shown for representative sections of the lungs and liver in the different groups. Nuclear stain with 4′,6-diamidino-2-phenylindole in blue. One representative experiment of 3 is shown.