Global analysis of the platelet palmitoylome. (A) Graphical depiction of the 1300 proteins identified in resting platelet membranes. Gray dots represent identified proteins not meeting the criteria for significance. Candidate palmitoyl proteins (○) cluster around the x-axis with known palmitoylated proteins and putative palmitoyl proteins identified in other palmitoylation-specific proteomic studies (green dots). Also shown are well-established palmitoylated platelet proteins identified (blue dots) and not identified (red dots) as being palmitoylated in this study. Inset: Expanded view of the graph for proteins with < 50 spectral counts. Diagonal line in each graph indicates the HA⁺/HA⁻ cut-off. (B) Western blotting analysis of ABE-purified proteins from platelet membranes, as prepared for proteomic analysis, in the presence and absence of HA using antibodies directed against Cdc42. Also shown is the total protein input for the HA⁺ and HA⁻ samples.