In vitro phenotypes of sorted hematopoietic populations from Rps6 mutant animals. (A) Photomicrographs (top) of a representative nonmegakaryocyte (left) and megakaryocyte (right) colony derived from a single HSC after 5 days in liquid culture. The percentage of wells that differentiate into megakaryocyte colonies at days 3 and 5 is shown for Tg.MxCre/+ and Rps6lox/+;Tg.MxCre/+ animals (bottom, n = 5 animals per genotype, at least 24 wells were evaluated per animal). (B-D) Photomicrographs (top) and the mean number of megakaryocytes (bottom) derived from 10 MPP (B), PreMegE (C), and MkP (D) cells sorted into a single well. Colonies were evaluated on days 1, 2, and 3. (n = 24 wells per animal with 2 animals of each genotype examined). P values are based on a 2-tailed t test, *P < .05, ***P < .001; NS indicates not significant. Scale bars: 50μM (A), 150μM (B-D).
