NLI and complex members TAL1, GATA-1, and ETO2 differentially bind to the LCR and globin genes in low- and high-HbF cells. ChIP was performed using Abs specific for NLI (A), GATA-1 (B), TAL1 (C), and ETO2 (D) to determine occupancy in low- and high-HbF cells. Solid black bars indicate low-HbF cells; white bars, high-HbF cells. Dark and light gray bars indicate values for an isotype-matched control Ab in low- and high-HbF cells, respectively. The shaded area shows the 900-bp region encompassed by the 3 primer pairs within BGL3. The dotted line in all panels indicates the average of IgG control precipitations across all amplicons. All data were calculated and plotted as described in Figure 2D. *P < .05.