American Society of Hematology

Figure 1

$Figure 1. Substantial co-occupancy of genomic sites by NLI and GATA-1 in human cells. Localization of NLI, GATA-1, and RNA pol II in K562 cells was examined by ChIP-chip. (A) Venn diagram showing intersection of genomic binding sites of GATA-1, NLI, and pol II within the region probed by tiling array. Total sites are indicated in parentheses; some pol II sites overlap more than one GATA-1 or NLI site, preventing the sum of interactions in the pol II circle from being equal to the total sites observed. (B) Bar plot of fraction of sites for each factor within promoters, exons, introns, and intergenic space based on RefSeq annotations. Promoter regions are defined as TSS plus 1 kb upstream. Exon and intron categories are not mutually exclusive because of multiple isoforms and overlapping genes. Intergenic space is defined as a lack of annotated transcripts and excludes the 1-kb promoter regions. (C). Tiling array signal (log2 ratios) across the globin locus on chr11. Regulatory elements and globin gene promoters are indicated by dotted lines. Peaks were called by ACME (using the parameters described in “NL1/GATA-1/Pol II ChIP-chip”) and are indicated below each track as dark gray boxes. Results for NLI, GATA-1, and RNA pol II ChIP-chip are shown. Data have been deposited in GEO under accession number GSE30047.$

Substantial co-occupancy of genomic sites by NLI and GATA-1 in human cells. Localization of NLI, GATA-1, and RNA pol II in K562 cells was examined by ChIP-chip. (A) Venn diagram showing intersection of genomic binding sites of GATA-1, NLI, and pol II within the region probed by tiling array. Total sites are indicated in parentheses; some pol II sites overlap more than one GATA-1 or NLI site, preventing the sum of interactions in the pol II circle from being equal to the total sites observed. (B) Bar plot of fraction of sites for each factor within promoters, exons, introns, and intergenic space based on RefSeq annotations. Promoter regions are defined as TSS plus 1 kb upstream. Exon and intron categories are not mutually exclusive because of multiple isoforms and overlapping genes. Intergenic space is defined as a lack of annotated transcripts and excludes the 1-kb promoter regions. (C). Tiling array signal (log2 ratios) across the globin locus on chr11. Regulatory elements and globin gene promoters are indicated by dotted lines. Peaks were called by ACME (using the parameters described in “NL1/GATA-1/Pol II ChIP-chip”) and are indicated below each track as dark gray boxes. Results for NLI, GATA-1, and RNA pol II ChIP-chip are shown. Data have been deposited in GEO under accession number GSE30047.

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