Knockdown of Gfi1 in BMSCs from an MM patient rescues Runx2, Ocn, and Bsp expression. The BM aspirates from MM patients and normals were as described in Figure 4E. BMSCs from an MM patient and a normal were transfected with either siGfi1 or siControl (untransfected samples were also analyzed). RNAs were collected after transfection (day 0) and after 5 days in OB differentiation media (day 5) and analyzed by quantitative PCR for Gfi1, Runx2, Ocn, and Bsp expression levels. All samples were analyzed relative to the RNA level in the day 0 sample from the normal, and the day 5 data are presented. A 70% knockdown of Gfi1 was detected in BMSCs from both the MM patient and the normal. The Runx2, Ocn, and Bsp expression in the siGfi1 treated BMSCs from the MM patient was significantly increased relative to the siControl-treated BMSCs (P < .05). These data are representative of 2 MM patients analyzed.