mTORC2 is not essential for survival but is essential for proliferation of myeloproliferative HMC-1.2 MCs. (A) HMC-1.2 MCs were transfected and analyzed at day 7 after transfection as in Figure 6B. At day 7 after transfection, cell viability of the transfected cells was determined by annexin V assay (B), metabolic activity by MTT assay (C), cell expansion by evaluation of cell number increase after the 24 hours (D), cell proliferation by BrdU assay (E), and cell cycle analysis by flow cytometry (F-G). In panels A-G, data represent means and SEM (n = 3; 3 independent transfection), and differences between the cells transfected with control (nontarget) and each of rictor-targeted shRNAs are indicated (*P < .05 by t test).