mTORC2, but not mTORC1, is dispensable for the survival of huMCs. HuMCs were transfected with luciferase (nontarget) or 2 raptor-targeting shRNA vectors (Ra1 and Ra2; A), or 2 rictor-targeting shRNA vectors (Ri1, Ri2; B), and expression of the mTOR, raptor, and rictor proteins were determined by immunoblotting and evaluated at day 7 after transfection. Cell viability of the transfected cells was determined with the annexin V assay (C). Expression of raptor and rictor (D) and cell viability (E) was determined by, respectively, immunoblotting and annexin V assay at day 10 after transfection. In panels A through E, data represent means and SEM (n = 3; 3 donors) and differences between the cells transfected with control (nontarget) and the targeted shRNAs are indicated (*P < .05 by t test).