Role of mTORC1 and mTORC2 in proliferation and cell cycle of HMC-1.2 MCs. (A) HMC-1.2 MCs were supplemented with vehicle alone (Ctrl), mTORC1/mTORC2 (Torin1, 200nM), or mTORC1 (rapamycin, 200nM) inhibitors and cultured for 72 hours. Incorporation of BrdU was determined with the BrdU assay and evaluated. (B-C) HMC-1.2 MCs were supplemented with vehicle alone and inhibitors as in panel A, cultured for 72 hours, and subjected to cell cycle analysis by flow cytometry (B) and evaluated (C). In panels A and C, data represent means and SEM (n = 3; 3 independent sample preparations) and differences among the differentially treated samples are indicated (*P < .05 by 1-way ANOVA). In panel B, data are means and SEM (n = 3; 3 independent sample preparations).