Colocalization of stomatin with endosomal marker CD71. Confocal imaging of cultured late-stage control and sdCHC reticulocytes probed with anti-stomatin (green) and anti-CD71 (transferrin receptor, red). The z-stack of sdCHC and control reticulocytes was examined, and the number of internal vesicles in which stomatin appeared, or TfR appeared, or in which the proteins colocalized, was noted. On average control reticulocytes contained 3 stomatin-positive vesicles per cell (n = 55 cells examined), whereas sdCHC reticulocytes contained 6 stomatin-positive vesicles per cell (n = 27 cells examined). In control cells ∼ 30% of the stomatin-positive vesicles colocalized with transferrin receptor. In sdCHC cells ∼ 60% of the stomatin-positive vesicles colocalized with transferrin receptor.