Transcriptional suppression and methylation of PTPROt in lymphocytes from TCL1 Tg mice. Real-time RT-PCR analysis of RNA from CD19⁺ selected spleen B cells from WT and TCL1 Tg mice at CLL stage (A) and at 7 weeks (B). Error bars represent SD. P values were calculated using the Student t test. (C) Bisulfite converted DNA from spleen CD19⁺-selected B cells of WT and TCL1 Tg mice at 7 weeks (top) and at CLL stage (bottom) were subjected to combined bisulfite restriction analysis using HinfI as the restriction enzyme.