Fine-mapping of Tb2r1. (A) Schematic representation of the generation of SISCSs from congenic mice. (B) Map of telomeric region of chr.4 in B6.D2-chr.4 mice and SISCS. DFPSNP indicates distance from preceding SNP. (C) TGF-β2 response of LSK cells from SISCS in FLT3L, KL, and TPO, 50 ng/mL each, 10% serum (n > 3; P < .05). (D) SNP map of the C57BL/6-derived region shared between SISCS lines 9 and 13. (E) Structure of PRDM16 protein. PR indicates PR domain (SET); ZF, zinc finger domain; and NS-SNP, nonsynonymous SNP at aa 760. (F) DNA sequencing of the region surrounding rs32942538 in exon 9 of Prdm16 in C57BL/6 and DBA/2 mice. The left panel shows the alleles of rs32942538 in C57BL/6, DBA/2, B6.D2-chr.4, and several SISC lines.