Effect of TGF-β2 deficiency on STAT5, AKT, and ERK1/2 phosphorylation. (A) FLT3 expression on LSK cells from Tgfb2^+/− mice and wt littermates, as measured by flow cytometry (n = 8; P = .93; left). Data are presented as the ratio of FLT3-PE and isotype control IgG_2a-PE geometric mean fluorescence. (B) Representative example of flow cytometric analysis of phosphorylation of STAT5, AKT, and ERK1/2 in LSK cells from Tgfb2^+/− (gray) and wt littermate (black) mice. IgG controls are shown in dashed lines. (C) Intracellular phosphorylated STAT5 (pSTAT5; n = 6; P = .11), AKT (pAKT; n = 7; P = .06), and ERK1/2 (pERK1/2; n = 6; P = .02) levels in wt and Tgfb2^+/− LSK cells. Data are presented as the ratio of pAKT, pSTAT5, or pERK1/2 and isotype control geometric mean fluorescence. Lines connect data from Tgfb2^+/− and wt mice within the same litter and experiment. P values were calculated with Wilcoxon signed rank test for paired samples, not normally distributed.