Phosphoethanolamine/phosphocholine ratio in a TF1-ETNK1 cellular model. (A) Western blot anti-ETNK1 showing the intracellular level of ETNK1 protein in TF1-WT, N244S, and H243Y. (B) Intracellular phosphoethanolamine/phosphocholine ratio as assessed by HPLC–mass Spectrometry in TF1 cells transduced with ETNK1-WT, N244S, and H243Y.