TCR+CD3 co-transduction of T cells enhances tumor infiltration of T cells. Thy1.1+ CD8+ T cells from firefly luciferase+ transgenic C57BL/6 mice were transduced with F5-TCR or F5-TCR+CD3-GFP. Thy1.2+ C57BL/6 mice were sublethally irradiated (5.5 Gy) and injected subcutaneously with 1 × 106 EL4.NP tumor cells on day 0 followed by intravenous transfer of a total of 3 × 105 transduced luciferase+Thy1.1+ T cells the next day. (A) T-cell accumulation at the site of tumor growth was monitored by IVIS-100 bioluminescence camera at the indicated time points. (B) The graph shows the mean bioluminescent signals in photons/s in the groups of mice at the indicated time. (C) The photons/s were converted into number of accumulated T cells based on the bioluminescent signals obtained when titrated numbers of luciferase+ transgenic T cells were injected subcutaneously. Data are representative of 5 mice/group.
