Increased IL-6 secretion by CMV-MoDCs and inhibition of STAT5 phosphorylation by IL-6. Monocytes were differentiated into DCs by culture for 5 days with GM-CSF and IL-13 after mock-infection (Med), infection with live (CMV-MoDC), or UV-inactivated CMV (UVCMV). (A) MoDCs (Med) and CMV-MoDCs (CMV) were differentiated by culture for 5 days; their inflammatory cytokines were assayed with a dedicated Ab array (Raybiotech), and IL-6 was quantified by CBA. (B) Monocytes were also differentiated in the presence of recombinant IL-6. The percentages of cells expressing CD1a, CD1c, and CD83 were determined by flow cytometry. (C-D) Total and phosphorylated STAT5 (pSTAT5) were analyzed by Western blotting of cell lysates of IL-6–MoDCs (IL-6) and CMV-MoDCs (CMV) or CMV-MoDCs treated with anti–IL-6 neutralizing Abs (αIL-6; 15 μg/mL). The histograms below their respective Western blots represent the quantification of 3 independent experiments. Error bars indicate the SD. Representative of ≥ 3 independent experiments.