Msr1 over-expression causes a delay of CML development. (A) BCR-ABL and MSR1 were detected by protein blotting using antibodies against ABL and MSR1. MSR1 protein was detected in cells transfected with BCR-ABL-IRES-Msr1-pMSCV. (B) Kaplan-Meier survival curves for recipients of BCR-ABL-IRES-pMSCV–transduced bone marrow cells (n = 7) or BCR-ABL-IRES-Msr1-pMSCV–transduced bone marrow cells (n = 7) donor mice. FACS analysis showed that the total number (C) and percentage (D) of Gr-1⁺ cells in peripheral blood of recipients of BCR-ABL-IRES-pMSCV–transduced bone marrow cells or BCR-ABL-IRES-Msr1-pMSCV–transduced bone marrow cells (*P < .05, **P < .01). Recipient of pMSCV-GFP–transduced bone marrow cells was shown as a control.