NLC- and BCR-induced secretion of the chemokines, CCL3, CCL4, and CXCL13 by CLL cells is inhibited by CAL-101. (A) The bar diagram represents the mean supernatant concentrations of CLL3 and CCL4 from CLL cells cultured in complete medium (control), medium supplemented with 10 μg/mL of anti-IgM, or anti-IgM and various concentrations of CAL-101. Displayed are the mean (± SEM) supernatant concentrations from 5 different patient samples assessed after 24 hours. The secretion of CCL3 and CCL4 from CLL cells in the presence of anti-IgM mAbs was significantly inhibited by CAL-101, with P < .05, as indicated by the asterisks describing the comparison of results from each culture containing CAL-101 to the results from the culture containing anti-IgM alone. (B) This bar diagram represents the mean CLL cell supernatant concentrations for CCL3 and CCL4 from CLL cells cocultured with or without (controls) NLCs. Displayed are the means (± SEM) from 5 different patient samples, assessed after 24 hours. The secretion of CCL3 and CCL4 from CLL cells was significantly inhibited by CAL-101, with P < .05, as indicated by the asterisks, describing the comparison of results from each culture containing CAL-101 to the results from the control culture. Lower concentrations of CAL-101, which are indicated next to each bar diagram and depicted by different shades of gray, also significantly reduced CCL3 (C) and CCL4 (D) concentrations in CLL-NLC cocultures (n = 3). (E) This bar diagram represents mean (± SEM) supernatant CXCL13 concentrations from CLL cells cultured alone or cocultured with NLC from 5 different patients, assessed after 24 hours. The secretion of CXCL13 was reduced by CAL-101, with P < .05, as indicated by the asterisks describing the comparison of results from each culture containing CAL-101 to the results from control cultures.