Tyrosine nitration of CD40 affects CD154-mediated TRAF protein recruitment. (A) Urate (10μM, 30-minute preincubation) and (B) methyl-β-cyclodextrine (MCD; 5μM, 30-minute preincubation) reverse the peroxynitrite (160μM, 15 minutes) mediated dissociation of TRAF2 and TRAF6 from CD40 in CD154-stimulated endothelial cells under static conditions (20-minute incubation). Statistical summary (n = 3; *P < .05 vs CD154; #P < .05 vs CD154 + peroxynitrite) and exemplary Western blot analyses of TRAF2 and TRAF6 coimmunoprecipitating with CD40.