Expression profiling in R174Q and R139X RUNX1 mutated patients CD34⁺ cells. Expression fold-change analysis of genes selected from array data (supplemental Table 2) in independent samples by Q-PCR. RNA was directly extracted and reverse-transcribed from sorted CD34⁺ cells obtained from AII-1, AII-2 (R174Q mutation) and BI-1, BII-2 (R139X mutation) patients and healthy donors peripheral blood. Gene expression was normalized to 18S and results are expressed as fold changes related to healthy control.