Xenotransplantation of RUNX1 R174Q CD34⁺ cells into irradiated NOG mice. FPD/AML CD34⁺ cell capacity for hematopoietic reconstitution in a xenotransplantation model. Human cell engraftment for 2 normal donors (C) and for 2 patients with R174Q mutation (AII-1 and AII-2) was estimated at week 15 by flow cytometry as percentage of human CD45⁺ (A) or CD33⁺ (myeloid) among CD45⁺ cells (B) in the BM. Mean ± SD of 2 mice per donor and patient.